ABSTRACT
Objective: To prove the effectiveness and feasibility of a paclitaxel hirudin complex and to provide experimental data on the prevention of restenosis, we investigated the effects of paclitaxel hirudin complexes on the growth of human coronary artery smooth muscle cells (HCASMCs) and endothelial cells (HCAECs) in vitro.
Methods: HCASMCs and HCAECs were co-incubated with different concentrations of hirudin. Cell viability was assessed using methylthiazoletetrazolium (MTT) assays to determine the optimal concentration range for inhibiting the growth of HCASMCs but not that of HCAECs. Then, cells were incubated with hirudin within the optimal concentration range combined with 1 μmol/L paclitaxel.
Results: Hirudin at 0.2-3.13 mg/mL significantly inhibited the growth of HCASMC (P<0.05) but not HCAEC (P>0.05) compared to the control group. This range of hirudin complexed with 1 µmol/L paclitaxel noticeably inhibited the growth of HCASMC (P<0.05). Moreover, 1 μmol/L paclitaxel+0.39 mg/mL hirudin noticeably decreased the inhibition ratio of the growth of HCAECs compared with the paclitaxel only group (P<0.05).The complex of 1 μmol/L paclitaxel plus 0.39 mg/mL hirudin can maximize the inhibition of HCASMCs and minimum the inhibition of HCAECs.
Conclusions: The results of this study may provide reference data for the subsequent development of natural herb-eluting stents.
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