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Use of Fusarium oxysporum Schl. cucumerinum Transformed with Green Fluorescent Protein to Follow Infection Patterns in Three Cucurbit Plants

DOI: 
10.7727/wimj.2015.184

ABSTRACT

Fusarium oxysporum Schl. is a soil-borne pathogen fungus, which could cause plant wilt diseases and caused great economic losses of agricultural production all over the world. However, the species of melon wilt diseases are too complex to breed stable disease-resistant melon strain. Therefore, exploring the main specialized differentiation factors of cucurbit wilt pathogen is important for elucidating the pathogenesis. In this study, we constructed green fluorescent protein (GFP)-labeled Fusarium oxysporum strain FSY0957, which was isolated from cucumber wilt. The GFP-labeled strain was inoculated in the roots of cucumber, muskmelon, and watermelon after 48 hours of observing the infection features of this strain. The results showed that the colonization ability of GFP-labeled strain to the cucumber plant was higher than that to muskmelon and watermelon. We established a genetic transformation system to investigate the infection mechanism of wither pathogens and colonization of plant rhizosphere.

Accepted: 
29 Apr, 2015
Weight: 
0
e-Published: 22 Jun, 2015

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