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Effect of Microencapsulated Hepatocytes Transplantation on Copper Metabolism of Rat Model with Hepatolenticular Degeneration

Journal Authors: 
DOI: 
10.7727/wimj.2015.433

ABSTRACT

Objective: To observe the effect of microencapsulated hepatocytes after intraperitoneal transplantation of  hepatolenticular degeneration (HDL) of model rats’ serum copper and

copper metabolism in liver. 

Methods: Rat HLD model was made by copper load diet. To prepare microencapsulated hepatocytes by the airflow method, and the method of intraperitoneal transplantation were used with naked cells or the microencapsulated cell transplantation, respectively, compared with blank control group group I), model group(group II), Bare hepatocytes after intraperitoneal transplantation group (group III) and microencapsulated hepatocyte transplantation group(group IV). Determination of HLD rat model of ALT, AST, albumin levels, copper of liver tissue, copper level in serum. 

Results: II, III, IV groups of rats at each time point of ALT, AST, serum copper, liver copper values were significantly higher than group I rats increased (each P < 0.05), synthesis of albumin levels decreased significantly (each P < 0.05).

Conclusion: Microencapsulated hepatocytes intraperitoneal transplantation can significantly reduce the liver copper deposition of HLD rat, accelerate metabolism of serum copper, may become a new method for cell transplantation in the treatment of HLD. The ALT, AST, serum copper and liver copper levels of group IV were significantly lower than that in group III after 7-14d (each P < 0.05), the lowest value respectively with 85.1±7.0 U/L, 87.9±22.7 U/L, 2.44±0.18μg/ml, 26.73±3.22μg/g, and albumin in 14d than that in group III, the most high up to 38.36±1.52g/L. 

Accepted: 
11 Aug, 2015
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e-Published: 18 Sep, 2015

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