Objective: To detect the skewness of TCR Vα and TCR Vβ of patients with Type 1 diabetes (T1D).
Methods: The heparinized venous blood was collected from ten patients with T1D. The peripheral blood lymphocytes (PBL) were isolated and used to extract mRNA. Reverse amplyfication was performed for cDNA synthesis.The skewness of TCR Vα and Vβ was detected with real-time florescence quantitative polymerase chain reaction (RQ-PCR) and analyzed by DNA melting-curve analysis technique, respectively.
Results: Among the TCR Vα genes, the skewness frequency rate (SFR) of Vα22 was 30%; both of Vα5 and Vα24 were 20%; the SFR of Vα28 was 10%, and which was the only gene showed restricted-clone. In all the Vβ genes, Vβ7 and Vβ17 were the the highest expression genes and their SFRs were both 60%. Vβ11 was near them with the SFR of 40%; the restricted clonal genes were Vβ18 and Vβ20, their SFRs were 10% and 20%, respectivley.
Conclusions: There are skewed genes inTCR Vα and TCR Vβ, which are probably relative to the onset of T1D.
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