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Isolation and Screening of Actinomycetes for Antimicrobial Activities from Marine Soil Sediments and Sponges

Journal Authors: 
DOI: 
10.7727/wimj.2016.194

ABSTRACT

Objective: The aim of this study was to isolate and screen for the antimicrobial properties of actinomycetes from marine soil sediments and sponges.

Methods: The samples of sponges and sediments were collected from various locations from the coast of Andhra Pradesh. Isolation of actinomycetes was done by plating them on starch casein agar, kuster’s agar, Actinomycete isolation agar and potassium tellurite agar medium using dilution technique. Various pathogenic bacterial strains, fungal strains and yeasts were obtained from Microbial Type Culture Collection (MTCC), Chandigarh, India and National Collection of Industrial Microorganisms (NCIM), Pune, India.

Results: A total of 6 different soil sediments were collected from the coast of Andhra Pradesh at depths ranging from 1 to 20 m. From the 9 different samples collected, 22 colonies of actinomycetes were isolated. Well separated and pure colonies of 12 actinomycetes were selected. Of these, 9 actinomycetes were active against Gram positive bacteria, 3 against Gram negative bacteria, 4 against filamentous fungi, and 1 against yeast. The crude extracts prepared from 3 potential isolates showed exhibited both antibacterial and antifungal activities. On the basis of morphological, physiological and biochemical characteristics, the most effective isolate identified (AP 13) as belonging to the genus Streptomyces.

Conclusion: Careful evaluation using previously published species of the genus Streptomyces, the isolate was identified as the strain Streptomyces fradiae.

Accepted: 
15 Jun, 2016
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e-Published: 29 Jun, 2016

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