ABSTRACT
Objectives:Esophageal cancer is a poorly characterized deadly cancer with a malignancy ratio of 2/3 among Iranian males and females, respectively. Recent studies on miR-520c and miR-519d have evidenced their either oncogenic or tumor suppressing roles in different cancers. The goal of this study was to assay the altered expressions of miR-520c and miR-519d in esophageal tumor tissues and adjacent non-tumor tissues in patients suffering from esophageal cancer.
Methods:RNA was extracted from 36-pair paraffin-embedded tumor and adjacent non-tumor tissues using TRIZOL kit and relative expressions of miR-520c and miR-519d were quantified by Real Time and RT-PCR using specific predesigned primers. CT method (2-ΔΔCT) and SPSS16 were used for statistical calculations.
Results:Statistical analysis has revealed spectacular upward expression increases of 10.4 and 24.5 folds of miR-520c and miR-519d adjacent in Esophageal Squamous Cells compared to their non-tumor tissues (P <0.05).
Conclusion:In general, the present study being the first report on the evaluation of miR-520c and miR-519d expressions in ESCC, is able to highlight the oncogenic roles of the two miRNA in ESCC and introduce them as appropriate potential alternatives to be utilized for further research on clinical treatment.
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