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Effect of 1,25-Dihydroxy Vitamin D3 on the Expression of Interleukin-17 and -21 in an Asthma Mice Model

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Objective: To explore the effect of 1,25-dihydroxy vitamin D3 (1,25(OH)2D3) on the expression of interleukin (IL)-17 and IL-21 in asthma by establishing an asthma mice model.

Methods: Forty female Kunming mice were randomly divided into normal control group (group A, n = 10), asthma group (group B, n = 10), 1,25(OH)2D3 intervention group (group C, n = 10) and dexamethasone treatment group (group D, n = 10). Asthma mice model was established and subsequently treated with different intervention. The airway lesions in mice were observed and the serum IL-17, IL-21 and immunoglobulin E (IgE) levels were determined using the enzyme-linked immunosorbent assay (ELISA) method. Immunohistochemical method was used to detect the expression of IL-17 and IL-21 in lung tissues.

Results:Serum IL-17 and IgE levels in groups B, C and D were remarkably higher than those in group A, especially in group B (F = 31.276, 18.677, p < 0.01). However, serum IL-21 level in group A was the highest, significantly higher than the other three groups (F = 22.406, p < 0.01). Immunohistochemical test revealed that the expression of IL-17 in group B was significantly higher than that in groups A, C and D, while IL-21 was strongly expressed in groups A and D but weakly expressed in group B.

Conclusion: In the pathogenesis of asthma, 1,25(OH)2D3 intervention can reduce the expression of IL-17 but elevate the expression of IL-21, suggesting it has a certain effect on asthma.

March 25, 2015
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