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Investigation of CTLA-4 and CD28 Gene Polymorphisms in Patients with Diabetes Mellitus Type 2 Using PCR-RFLP in a Turkish Population



Objective: The aim of this study is to investigate whether specific polymorphisms in the CTLA-4 and CD28 gene are associated with Type 2 diabetes mellitus (T2DM).

Methods: Blood samples were collected from 241 individuals (72 patients with T2DM and 169 healthy individuals) and DNA was isolated. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to detect the frequencies of CTLA-4 NM_005214.3:c.49A > G and c.-319C > T, and CD28 NM_006139.1:c.534+17T > C polymorphisms in T2DM patients in the Sanliurfa Population.

Results: The data suggested that body mass index (BMI), total cholesterol (TC), triglyceride (TG), lowdensity lipoprotein cholesterol (LDL-c) and haemoglobin A1c (HbA1c) were significantly higher in T2DM patients than in the control individuals (p < 0.05). No significant differences were observed for the frequencies of c.49A > G, c.-319C > T genotype and allele of CTLA-4 gene and c.534+17T > C of the CD28 gene in T2DM patients compared to healthy individuals (p > 0.05).

Conclusion: The CTLA-4 gene c.49A >G and c.-319C >T and CD28 gene c.534+17T > C polymorphisms did not represent an important risk factor for this disease in a group of the Turkish population.

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e-Published: 07 Oct, 2013
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