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Knockdown of ET-1 Gene Can Inhibit the Proliferation, Invasion of Human Prostate Cancer Cell

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Prostate cancer is the sixth most common fatal cancer and the second most frequently diagnosed cancer of male worldwide. Endothelin-1 (ET-1), generated by the prostate epithelia, plays a vital role in the genesis and the development, of prostate cancer. ET-1 has been previously reported in prostate and other organs. However, ET-1 expression in Prostate cancer cell lines has not been described. Therefore, we have used Western Blot to demonstrate the expression level of ET-1 in PC3 cell line. The results suggest the expression of ET-1 is significantly high expressed in PC3 cell line. Moreover, knockdown of ET-1 gene with siRNA can effectively repress the cell proliferation and the cell cycle was blocked in G1/G2 phase. Knockdown of ET-1 gene can affect cell invasion, which is confirmed by cell invasion and migration related genes using west blot (β-catenin, E-cadherin, RhoC and RhoA). The results of this study can describe the characterization of ET-1in the development of primary human Prostate cancer, which can lead to a better understanding of the changes occurring at the molecular level during the development and progression of cancer. 

13 Feb, 2017
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e-Published: 03 Mar, 2017


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