ABSTRACT
Objective: Slow coronary flow (SCF) is specified by the delayed passage of contrast in the absence of obstructive epicardial coronary disease. Its etiopathology remains unclear. Kinins are mediators of vasodilatation as well as inflammation in human body. This study aimed to investigate the gene expression of kinins B1 and B2 receptors (B1R and B2R) in peripheral blood mononuclear cells (PBMC) from patients with SCF.
Methods: Thirty patients with SCF (22 male/ 8 female; age: 53 ± 11.83 years) and 30 healthy controls (22 male/ 8 female; age: 51.37 ± 11.89 years) were enrolled for the study. Patients and controls were matched with age, gender and body mass index. Peripheral blood mononuclear cells (PBMC) were obtained for mRNA extraction. To analyze the gene expression levels of B1R and B2R, mRNA extraction, cDNA synthesis and quantitive reverse transcriptase polymerase chain reaction (QRT-PCR) were carried out for control and SCF groups.
Results: B1R expression showed statistically significant difference between SCF patients and controls (0.15 ± 0.03 vs. 0.98 ± 0.15 fold, respectively; p < 0.0001), SCF patients with 3 slow flow coronary arteries and controls (0.15 ± 0.04 vs. 0.98 ± 0.15 fold, respectively; P = 0.001) and SCF patients with <3 slow flow coronary arteries and controls (0.14 ± 0.04 vs. 0.98 ± 0.15 fold, respectively; p = 0.001). There was no found statistically significant difference between SCF (0.58 ± 0.11 fold) and control groups (1.22 ± 0.28 fold) in analysis of B2R gene expression (p = 0.143).
Conclusion: It can be concluded that decreased B1R gene expression and its signaling pathway may provide a structural basis of the important role of kinins in SCF pathogenesis.
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