Objective: To build an osteopontin (OPN) specific short interfering ribonucleic acid (siRNA) expression vector and transfect it into cervical cancer Hela cells, in order to obtain OPN stable transfection cell lines, thus providing support to the further study on the mechanism of OPN in cervical cancer Hela cells.
Methods: An OPN specific siRNA expression vector was bulit. Then the target fragment was connected to pcDNA3.1 (+) carrier and mediated with liposomes to transfect Hela cells, in order to obtain the RNA interference stable cell line. The OPN messenger RNA (mRNA) and protein relative expression in the OPN-siRNA group (transfected with the OPN specific siRNA), RANDOM group (transfected with non-interference plasmid) and pcDNA3.1 group (transfected with empty plasmid) were detected with reverse transcription polymerase chain reaction and Western blotting methods.
Results: The OPN mRNA and protein expressions were decreased significantly in the OPNsiRNA-1 group (p < 0.05).
Conclusion: The construction of OPN interference cervical cancer Hela cell line can provide a basis for the further study on the mechanism of the growth, metastasis and invasion of OPNmediate cervical cancer cells.