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Beta-lactamase Enzymes of Clinical Pseudomonas aeruginosa Strains

Issue: 
DOI: 
10.7727/wimj.2014.362
Pages: 
40–5

ABSTRACT

Objectives: In this study, the production of extended spectrum beta-lactamase (ESBL), metallo-beta-lacatamase (MBL) and AmpC beta-lactamase enzymes of Pseudomonas aeruginosa (P aeruginosa) strains which were isolated from clinical samples were investigated. AmpC gene was also detected by the polymerase chain reaction (PCR) analysis.

Methods: A hundred strains of P aeruginosa were included in the study. The presence of ESBL was investigated with combined disk confirmation test, MBL was investigated with E-test method and AmpC beta-lactamase was investigated with disk induction test. In order to detect the production of AmpC beta-lactamase genotypically, the PCR method was used.

Results: Only one strain was found to be MBL positive. Four per cent of strains were found to be ESBL positive. AmpC beta-lactamase production was positive in 73% of the strains with disk induction test. AmpC gene was detected in 96% of the studied strains with the PCR method.

Conclusion: While ESBL and MBL rates in this study were significantly lower than those found in other studies, the rate of AmpC beta-lactamase was higher. Although AmpC gene was detected in some strains (23%), they were not found  to produce AmpC beta-lactamase with disk induction test. 

 

 

Accepted: 
12 Dec, 2014
PDF Attachment: 
e-Published: 16 Jun, 2015
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