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Cytokine-induced Killer Cells Co-cultured with Dendritic Cell Benefit for the Treatment of MCF-7/ADR-Mammary Cancer with Multi-drug Resistance

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Cytokine-induced killer cells (CIK) are capable of lysing tumor cell and recognizing these cells in a non-MHC restricted fashion. After they were co-cultured with Dendritic cells (DC) loaded multidrug resistance tumor antigen, do they have the higher antitumor effect to the same multidrug resistance tumor?

Methods: Thetumor-bearing mice model was established by MCF-7/ADR. DC and CIK were respectively cultured from peripheral blood mononuclear cells (PBMC) derived from healthy individuals. MCF-7/ADR was prepared to obtain the antigen lyses. CIK was co-cultured with DC pulsed or unpulsed by the tumor antigen lyses (AP-DC+CIK and DC+CIK). So there were AP-DC+CIK, DC+CIK and CIK groups, NS was used as control group. The tumor-bearing mice were injected by intravenous with the above 4 groups’ effector cells. This study evaluated and compared the antitumor effect of different groups through the secretion of cytokines, the tumor size and the apoptosis of tumor cells in TUNEL test.

Results: After CIK co-cultured with DC, the frequency of CD3+CD56+ surface marker and the secretion of IFN-gamma, TNF-α and IL-2 were increased. After co-cultured, DC expressed the higher levels of mature markers, and secreted the more IL-12. The tumor size was the most inhibited in AP-DC+CIK group than in other 3 group. The TUNEL test had the significant differences between every two groups of 4 groups.

Conclusion: After CIK cells were co-cultured with DC loaded with multidrug resistance tumor antigen, the higher antitumor immune response was evaluated to multidrug resistance tumor expressed the same tumor antigen, which provide the effective clinical treatment to multidrug resistance tumor.

11 Sep, 2015
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e-Published: 03 Nov, 2015


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