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Detection of HPV Infection in Non-cancer Oesophageal Lesions and Normal Tissue by Nested Polymerase Chain Reaction



Objective: Human papilloma virus (HPV) can be the aetiologic factor in the benign or malignant oesophageal epithelium. HPV is suspected of causing extragenital cancers, including cancers of the oral cavity, larynx, oesophagus and the lungs. More than 40 genotypes of HPV that infect the anogenital area are associated with a large spectrum of diseases from benign proliferation to invasive cancers. The aims of our study were to evaluate the prevalence of HPV infection in non-cancer oesophageal lesions in Mazandran, North of Iran and identify the prevalence of HPV in benign lesions.

Methods: A total of 104 non-cancer oesophageal samples were collected in parafinated blocks of tissue archived in pathology. After deparafinated and DNA extraction, nested PCR (nPCR) method was performed by HPV L1 primer pairs (MY09/MY11 and Gp5+/GP6+).

Results: Of 104 cases, 35 (33.7%) were HPV DNA positive. By histopathology, 61.5%, 31.8%, 30.4% and 30% of dysplasia, oesophagitis, normal tissue and ulcerative lesion were positive to HPV DNA, respectively. Also, lower third (distal) of oesophagus were the less infection of HPV DNA (18.8%) than to upper and middle third (about 46%).  The most prevalence of HPV DNA found in > 75 years old cases.

Conclusion: To our knowledge, considering the highest rate of HPV infection in people more than 75, we recommend the diagnosis procedure will be done at lower age. Also, proximal part of oesophagus is more infected with HPV than distal part.

05 Jan, 2015
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e-Published: 21 Jul, 2015
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