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nested PCR

Detection of HPV Infection in Non-cancer Oesophageal Lesions and Normal Tissue by Nested Polymerase Chain Reaction

Issue: 
DOI: 
10.7727/wimj.2014.322
Pages: 
496-99
Synopsis: 
Human papilloma virus (HPV) can be the aetiologic factor in the benign or malignant oesophageal epithelium. Nested PCR was performed for detection of HPV in non-cancer oesophageal lesions. A third (33.7%) of samples were infected with HPV DNA. This can lead to cell changes that over many years may develop into cancer.

ABSTRACT

Accepted: 
05 Jan, 2015
PDF Attachment: 
Journal Sections: 
e-Published: 21 Jul, 2015

Cytomegalovirus Identification in Blood and Urine of Newborns by Nested Polymerase Chain Reaction

Issue: 
DOI: 
10.7727/wimj.2014.378
Pages: 
291–4
Synopsis: 
Identification of cytomegalovirus in newborns (congenital) by molecular biology methods polymerase chain reaction was more feasible using urine collection which is less invasive. The procedure, though laborious, is less expensive and more efficient compared to the use blood or blood culture.

 

ABSTRACT

Introduction: Knowing the frequency of congenital cytomegalovirus (CMV) infection in newborn admitted to the Division of Neonatology, analysed by nested polymerase chain reaction (PCR) and DNA detection differences in blood and urine specimens.

Methods:  The study was carried out for eight months. Newborns (n = 520) hospitalized in five hospitals in Campo Grande-MS were CMV checked. 

Accepted: 
12 Dec, 2014
PDF Attachment: 
Journal Sections: 
e-Published: 07 May, 2015
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