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Presence of Human Papillomavirus and Epstein–Barr virus in Squamous Lesions of the Tongue



Background: Several studies have suggested a possible role of human papillomavirus (HPV) and Epstein-Barr virus (EBV) in the pathogenesis of oral premalignant lesions. This study aimed to investigate the correlation between squamous dysplasia of the tongue and expression of p16 and Ki67 immunohistochemically as well as HPV genotypes with Real-time PCR.

Methods: Twenty-three tongue biopsies were detected with immunohistochemically for p16, EBV and Ki67 and Real-time PCR and chromogenic in-situ hybridization for HPV.

Results: Twenty-three tongue biopsy specimens diagnosed as pre-neoplastic and suspicious lesions for dysplasia (n:17) and HPV infection (n:6) microscopically, have no invasive carcinoma. Chromogenic in-situ hybridization for HPV DNA (HPV-III family16), There was no immunoreactivity for EBV. p16 positivity was found in 25% of dysplasia. One lesion was positive for HPV by Chromogenic in-situ hybridization and one case was positive by Real-time PCR for HPV.

Conclusions: The evidence suggested that HPV infection plays a role in the pathogenesis of squamous dysplasia localized tongue, but not EBV infections.

24 Aug, 2015
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e-Published: 10 Mar, 2016


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