Close Menu

Detection of OXA Carbapenemase Positive Acinetobacter spp., Jamaica

DOI: 
10.7727/wimj.2016.065

ABSTRACT

Objective:  The global problem of resistance to carbapenem antibiotics, through the production of carbapenemases by clinically significant bacteria, continues to increase while options for antibiotic therapy remain limited. It is important to determine the mechanism of carbapenem resistance in the multidrug resistant (MDR) Acinetobacter spp.  isolated at a tertiary care hospital, Jamaica (TCHJ) because of the implications for therapy which is the goal of this study.

Methodology: 82 MDR Acinetobacter spp collected at a TCHJ were identified as potential carbapenemase producers during routine susceptibility testing for a one year period from May 2009 to April 2010. These isolates were subjected to phenotypic and genotypic tests for carbapenemase detection using the modified Hodge test and PCR respectively. Multiplex PCR for OXA-23, -24, -51, -58 was performed and PFGE was used to determine if carbapenemase-positive isolates were related.

Results: PCR for OXA-carbapenemases found 13 blaOXA-24, two blaOXA-23 and one blaOXA-58, establishing a prevalence of 19.5%. PFGE results showed that Acinetobacter spp sharing phenotypic and genotypic similarites were clonally related.

Conclusions: Carbapenemase production was found to be a cause of antibiotic resistance in MDR Acinetobacter spp. isolates at a TCHJ. These isolates were thought to have been derived from an outbreak or endemic strain whose presence is likely to significantly impact patient management and antibiotic policy.

Accepted: 
20 Apr, 2016
PDF Attachment: 
e-Published: 27 Apr, 2016

Disclaimer

Manuscripts that are Published Ahead of Print have been peer reviewed and accepted for publication by the Editorial Board of the West Indian Medical Journal. They may appear in their original format and may not be copy edited or formatted in the style guide of this Journal. While accepted manuscripts are not yet assigned a volume, issue or page numbers, they can be cited using the DOI and date of e-publication. See our Instructions for Authors on how to properly cite manuscripts at this stage. The contents of the manuscript may change before it is published in its final form. Manuscripts in this section will be removed once they have been issued to a volume and issue, but will still retain the DOI and date of e-publication.

Top of Page