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Short-term Administration of Gonadotropin-releasing Hormone Agonist (Buserelin) Induces Apoptosis in Rat Ovarian Developmental Follicles



Objective: The aim of the present study was to investigate the effects of buserelin on the development of follicles, apoptosis index, and steroid hormones level.

Methods: Twenty four 3 month old female rats were randomly divided into three groups, that is, a low dose group, a high dose group and a control group (n=8). Buserelin and normal saline were injected subcutaneously for five days. Thirty days after the first injection, the ovaries were removed for staining. Moreover, blood samples were collected and centrifuged. Their serum was used for measuring estradiol and progesterone levels using Enzyme-Linked Immunosorbent Assay (ELISA).

Results: The findings revealed a significant decrease in the mean of secondary and graffian follicles in the high dose group as compared with the control group (P=0.037, P=0.034, respectively). The serum estradiol level increased significantly in the high dose group as compared with the low dose and control groups (P=0.027, P=0.047, respectively). The serum progesterone level decreased, although not significantly. In contrast to the control group, the significant increase of apoptotic cell death was found in primordial, unilaminar and multilaminar follicles in high dose group (P=0.004, P=0.049, P=0.047, respectively).

Conclusion: The findings of this study suggest that short-term administration of high dose buserelin increases the serum estradiol level and apoptosis in the granulosa cells but has an inhibitory effect on follicular development.

15 Jun, 2016
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e-Published: 30 Jun, 2016


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