Close Menu

Histopathological and Immunohistochemical Study of the Effect of Sildenafil Citrate, Vitamin A, Vitamin C and Vitamin E on Wound-healing in Alloxan-induced Diabetic Rats



Objectives: This experimental study examined the impact of sildenafil citrate, vitamin A (Vit A), vitamin C (Vit C) and vitamin E (Vit C) on wound healing in a rat model of diabetes (n=100).

Materials and Methods: Diabetes was induced in the rats by intraperitoneal (i.p.) administration of alloxan. After anaesthesia, a standard wound was created on the back of each rat using a 10-mm sterile punch. The rats were randomly divided into 10 groups (n=10 in each), 1–10, as follows: normal saline, glibenclamide, insulin, sildenafil, Vit A, Vit C, Vit E, Vit A + sildenafil, Vit C + sildenafil, Vit E + sildenafil daily for 15 days. The rats were sacrificed after being anesthetised 3, 7 and 15 days later. Wounded skin tissue samples were collected for histopathological and immunohistochemical analyses.

Results: On the seventh day, epithelial regeneration was completed in Groups 8 and 9. Angiogenesis was insufficient in Group 2. In terms of connective tissue proliferation, partially matured connective tissue was observed in Group 4. On the 15th day of the study, Groups 8, 9 and 10 had mature connective tissue. However, Group 1 still had exudate-containing neutrophils. Immunohistochemically, on the third day, the level of inducible nitric oxide synthase (iNOS) reactivity was intense in macrophages and neutrophils surrounding the wound edges in Groups 4, 8, 9 and 10. The level of iNOS reactivity was moderate in Group 6 and less distinct in Groups 1, 2, 3, 5 and 7.

Conclusion: Sildenafil citrate, together with Vit A and Vit C, is beneficial in wound healing of diabetics.

07 Jan, 2016
PDF Attachment: 
e-Published: 16 Mar, 2017


Manuscripts that are Published Ahead of Print have been peer reviewed and accepted for publication by the Editorial Board of the West Indian Medical Journal. They may appear in their original format and may not be copy edited or formatted in the style guide of this Journal. While accepted manuscripts are not yet assigned a volume, issue or page numbers, they can be cited using the DOI and date of e-publication. See our Instructions for Authors on how to properly cite manuscripts at this stage. The contents of the manuscript may change before it is published in its final form. Manuscripts in this section will be removed once they have been issued to a volume and issue, but will still retain the DOI and date of e-publication.

Top of Page