Six-shogaol is a major constituent of the rhizome from the ginger plant. In this study, evidence that 6-shogaol significantly inhibited production of pro-inflammatory cytokines TNF-alpha, IL-1 beta and nitric oxide from lipopolysaccharide stimulated RAW 264.7 macrophages was obtained. Such inhibition might explain the anti-inflammatory mechanisms and the therapeutic potential of this ginger compound.