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DNA vaccine

Cloning and Constructing a Plasmid Encoding Leishmania Eukaryotic Initiation Factor Gene of Leishmania major Fused with Green Fluorescent Protein Gene as a Vaccine Candidate

Issue: 
DOI: 
10.7727/wimj.2014.201
Pages: 
256–9
Synopsis: 
The LeIF gene was cloned into pJET1.2/blunt vector and then it was subcloned into pEGFP-N1 in the upstream of EGFP Gene. The recombinant plasmid could be used as a vaccine candidate against leishmaniasis and EGFP is used as a reporter to detect this protein expression.

ABSTRACT

Objective: Leishmaniasis is usually treated by chemotherapy; however, toxicity, resistance and high-cost limits the use of the chemical drugs. Leishmania eukaryotic initiation factor (LeIF) protein acts the same as interleukin (IL)-12 and reduces the secretion of IL-4 in lymph node cells of mice infected with L major. The aim of this study was cloning of the gene encoding LeIF antigen into eukaryotic expression plasmid pEGFP-N1.

Accepted: 
09 Sep, 2014
PDF Attachment: 
Journal Sections: 
e-Published: 12 May, 2015
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