Using the modern WGA-HRP neurohistochemical tracing technique, the brainstem localization of vagal preganglionic parasympathetic neurons was investigated in the Agouti. Following injections of the tracer into the muscular wall of various sections of the stomach, retrogradely labelled neurons were seen in the dorsal motor nucleus of the vagus nerve as well as in the nucleus ambiguus. The labelled neurons were bilaterally distributed.

This study has investigated the organization of vagal brainstem nuclei in the agouti and has shown organisation pattern similar to those of other commonly used laboratory animals. The larger size of this species makes it a suitable alternative experimental animal.