Metals are central to hold the bioactivity of numerous enzymes and proteins on which life is based. Several previously published studies demonstrated the functions of transition and trace elements Fe, Zn, Mg, Cu, Mn and Cr in insulin action and carbohydrate metabolism. This study aimed to assess the interrelationship of some selected metals such as Mg, Zn, Cu, Cr, Mn, Pb, Ni and Fe in urines of T2D patients versus non-diabetic subjects. The findings of this study could provide a way to explore urinary metal-based marker(s) for disease pathogenesis and progression.